无菌室一般是在微生物实验室内专辟的一个小房间，面积不宜过大，约4m2~5m2，高2.5m左右即可。无菌室外要设一个缓冲间、净化风淋间及更衣间。无菌室和缓冲间都要密闭。室内装备要有空气过滤装置。无菌室和缓冲间都装有紫外线灯，无菌室的紫外线灯距离工作台面1m。无菌室内的地面、墙壁要平整，不易藏污垢，便于清洁。工作台的台面要处于水平状态。工作人员进入无菌室应在更衣室穿戴好灭菌的衣、帽、鞋，经过净化风淋室，再到缓冲间，然后进入无菌室进行检验工作。县级市的企业一般实验室多使用小型超净工作台。超净工作台其结构简单，便于移动，工作台内有紫外灯和净化风机，外部是推拉式小门或开两个洞的玻璃门，操作时将双臂伸进去即可。

The aseptic room is usually a small room specially set up in the microbiology laboratory. The area should not be too large, about 4m2 ~ 5m2, and about 2.5m high. A buffer room, clean air shower room and dressing room should be set up outside the sterile room. Sterile room and buffer room should be sealed. Indoor equipment should be equipped with air filtering device. The ultraviolet lamp is installed in the aseptic room and buffer room, and the ultraviolet lamp in the aseptic room is 1m away from the working table. The floor and wall of aseptic room should be flat, not easy to store dirt and easy to clean. The worktable should be in a horizontal state. When entering the sterile room, the staff should wear sterilized clothes, hats and shoes in the changing room, go through the purification air shower room, and then go to the buffer room, and then enter the sterile room for inspection. Small ultra clean worktable is often used in the laboratories of county-level enterprises. The ultra clean worktable is simple in structure and easy to move. There are ultraviolet lamps and purification fans in the working table, and the outside is a small push-pull door or a glass door with two holes. When operating, you can stretch your arms in.